Table 1. Data collection, Processing and Model refinement parameters.
| Parameter | Value |
|---|---|
| Data collection | |
| Voltage (kV) | 300 |
| Magnification (x) | 130000 |
| Å/pix | 1.06 |
| Energy Filter with Slit (eV) | 20 |
| Frames per Micrograph | 40 |
| Total Dose (e- /Å2) | 40 |
| Micrographs | 3220 |
| Defocus Range (μm) | 0.8–2.6 |
| Data Processing | |
| Micrographs | 3200 |
| Frames used | 4–40 |
| Å/pix | 1.06 |
| Particles (Total) | 46348 (56275) |
| Symmetry Applied | I4 |
| Overall Resolution@FSC0.143 (Å) | 2.75* |
| B-factor (Å2) | −104.93* |
| Model Refinement | |
| Composition | |
| Chains | 19 |
| Atoms | 41731 (Hydrogens: 0) |
| Refinement | |
| CCmask | 0.7961 |
| CCvolume | 0.7941 |
| ADP (B-factors) | |
| Iso/Aniso | 41731/0 |
| Mean | 101.95 |
| RMS deviations | |
| Bonds (Å) | 0.006 |
| Angles (O) | 0.806 |
| EMRinger Score | 5.40 |
| MolProbity validation | |
| Clash score, all atoms | 3.35 |
| MolProbity score | 1.57 |
| Rotamer Outliers (%) | 0.16 |
| Ramachandran | |
| Favoured (%) | 93.04 |
| Allowed (%) | 6.83 |
| Outliers (%) | 0.13 |
* Calculated by RELION.