Figure 5.

DHED treatment increases T:M ratio in 3K mice. a, Intact cell-crosslinking of αS in cortices of male and (c) female 3K and WT mice treated with either vehicle (Sham) or DHED. b, Higher or lower blot exposure time shows both increase in multimeric and decrease in monomeric αS. d, Quantitative WBs of αS monomers (∼14 kDa) and tetramers (∼60 kDa) reveal a trend to increase tetramer level and a significant reduction of monomeric αS level in 3K males and to similar level of 3K females (each normalized to WT condition). Separation of graphs due to blots being run at different time points. e, Stepwise increase in T:M ratio in DHED treated brain of either male or female 3K mice toward level of normal WT mice. Data are mean ± SEM. d, *p < 0.05 (two-tailed t test). e, *p < 0.05 (two-way ANOVA post Tukey).