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. Author manuscript; available in PMC: 2020 Mar 15.
Published in final edited form as: Cancer Res. 2019 Jul 23;79(18):4703–4714. doi: 10.1158/0008-5472.CAN-19-0868

Figure 6.

Figure 6.

Invasion is prevented by expression of uncleavable α6 integrin mutant (DU145 α6AA). Prostate tumor cells expressing the α6 integrin (DU145 α6WT solid bars) not expressing the α6 integrin (DU145 α6KO open bars) or a mutated and uncleavable α6 integrin (DU145 α6AA striped bars) were tested 16 hours later for their ability to invade through laminin containing Matrigel (laminin), a smooth muscle cell layer (muscle) or a collagen layer (collagen) using a modified Boyden chamber assay. The number of cells appearing on the underside of the insert were counted in triplicate wells. The standard error of the median values is shown and compared using a two-way ANOVA analysis (****p<0.000001). Results are representative of at least 3 biological and technical replicates; n=364 for DU145 α6WT, n=370 for DU145 α6KO, and n=378 for DU145 α6AA.