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. Author manuscript; available in PMC: 2019 Sep 18.
Published in final edited form as: Cell Rep. 2019 Aug 20;28(8):2080–2095.e6. doi: 10.1016/j.celrep.2019.07.069

Figure 2. Hsp104 NBD1 Variants Suppress TDP-43 Toxicity, Aggregation, and Mislocalization.

Figure 2.

(A) NBD1 variants suppress TDP-43 toxicity in yeast. W303aΔhsp104-pAG-303GAL-TDP-43 yeast were transformed with Hsp104 variants or vector. Strains were serially diluted 5-fold and spotted in duplicate onto glucose (non-inducing) and galactose (inducing) media.

(B) NBD1 variants do not reduce TDP-43 expression in yeast. Strains in (A) were induced for 5 h, lysed, and immunoblotted for Hsp104, TDP-43, and PGK (loading control).

(C) NBD1 variants suppress TDP-43 aggregation and mislocalization in yeast. Fluorescence microscopy of cells coexpressing fluorescently tagged TDP-43 and Hsp104 variants. Strains were induced for 5 h in galactose, fixed, and stained with DAPI (blue) to visualize nuclei. Scale bar, 2.5 μm.

(D) TDP-43 localization was quantified by calculating the proportion of cells containing colocalized nuclear staining. Values represent means ± SEM (n = 3).

See also Figures S1 and S2.