Skip to main content
. Author manuscript; available in PMC: 2019 Sep 18.
Published in final edited form as: Cell Rep. 2019 Aug 20;28(8):2080–2095.e6. doi: 10.1016/j.celrep.2019.07.069

Figure 6. Potentiated NBD1 Variants Display Enhanced ATPase, Disaggregase, and Unfoldase Activity.

Figure 6.

(A) NBD1 variants exhibit elevated ATPase activity. Values represent means ± SEM (n = 2–3).

(B) NBD1 variants exhibit elevated disaggregase activity. Luciferase aggregates were incubated with Hsp104 variant plus (gray bars) or minus (black bars) Hsc70 (0.167 μM) and Hdj2 (0.167 μM). Values represent means ± SEM (n = 2–7).

(C) NBD1 variants exhibit elevated unfoldase activity. RepA1–70-GFP was incubated with Hsp104 variant and GroELtrap plus ATP or ATP:ATPγS (1:1). GFP unfolding was measured by fluorescence. Representative data from three trials are shown.

See also Figures S4.