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. Author manuscript; available in PMC: 2020 Sep 1.
Published in final edited form as: Circ Genom Precis Med. 2019 Aug 28;12(9):407–420. doi: 10.1161/CIRCGEN.119.002641

Figure 7.

Figure 7.

NEDD4-1-dependent downregulation of LTCC by LITAF in 3-week-old rabbit cardiomyocytes. A, Protein levels of total Cavα1c, NEDD4-1, and tubulin in cells expressing scrambled control RNA or shRNA against endogenous NEDD4-1 (left panel; the asterisk indicates an unspecific band). Respective changes in NEDD4-1 and Cavα1c abundance, normalized to tubulin (n=5 animals, performed in triplicate; mean±SEM). Student’s t-test, p<0.05 (right panel). B, Mean current-voltage relationships of ICa,L peak currents for baseline conditions from cells expressing GFP and shRNA against endogenous NEDD4-1 (control) or LITAF and NEDD4-1 shRNA. C, 3wRbCM were transduced with adenovirus expressing scrambled RNA and LITAF (control) or LITAF and shRNA against endogenous NEDD4-1. Mean current-voltage relationships of ICa,L peak currents for baseline conditions from respective cells are depicted (cells from 5 animals; mean±SEM; Student’s t-test, p<0.05).