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. Author manuscript; available in PMC: 2020 Aug 1.
Published in final edited form as: J Neurovirol. 2019 Apr 24;25(4):508–519. doi: 10.1007/s13365-019-00751-0

Figure 3. Ezh2, H3K27me3, MeCP2, and Tat expression in relation to HIV infection and latency in astrocytes.

Figure 3.

Figure 3.

U373.MG and U373.MG.Tat were infected with VSVG-pseudotyped RGH viruses and analyzed every 3-5 days by Western blotting (A), followed by densitometry quantitation for Ezh2 (B), H3K27me3 (C), and MeCP2 (D), or by qRT-PCR for Tat mRNA expression from RGH (E) or in U373.MG.Tat (F) using RGH Tat mRNA-specific, or Tat.myc mRNA-specific primers. PCNA was included as a loading control and used as a quantitation reference for Western blotting. β-actin was used an internal control and used as used as a quantitation reference for qRT-PCR. All were expressed as relative levels (Rel.). The data were Mean ± SD of triplicates and representative of three independent experiments.