KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| APC CD49b anti-mouse mAb (DX5) | Thermo Fisher | Cat #17-5971-82 |
| eFluor660 CD107a anti-mouse mAb (1D4B) | Thermo Fisher | Cat #50-1071-82 |
| PerCP-eFluor710 EOMES anti-mouse mAb (Dan11mag) | Thermo Fisher | Cat #46-4875-82 |
| APC Granzyme B anti-human mAb (GB12) | Thermo Fisher | Cat #MHGB05 |
| PE IFN gamma anti-mouse mAb (XMG1.2) | Thermo Fisher | Cat #12-7311-82 |
| PE-Cyanine7 NKp46 anti-mouse mAb (29A1.4) | Thermo Fisher | Cat #25-3351-82 |
| Alexa Fluor 647 Stat5 (pY694) mAb (47/Stat5(pY694)) | BD Biosciences | Cat #562076 |
| Brilliant Violet 605 anti-mouse CD45 mAb (30-F11) | BioLegend | Cat #103139 |
| Brilliant Violet 605 anti-mouse CD45.1 mAb (A20) | BioLegend | Cat #110737 |
| PE anti-rat/mouse CD49a mAb (Ha31/8) | BD Biosciences | Cat #562115 |
| PerCP-Cy5.5 anti-mouse NK-1.1 mAb (PK136) | BD Biosciences | Cat #561111 |
| PE rat anti-mouse CD43 mAb (S7) | BD Biosciences | Cat #553271 |
| APC CY7 Hamster anti-mouse CD3e mAb (145-2C11) | BD Biosciences | Cat #557596 |
| BV605 Hamster anti-rat/mouse CD49a mAb (Ha31/8) | BD Biosciences | Cat #740375 |
| T-BET BV421 (4B10) | Biolegend | Cat #644815 |
| PE Rat anti-mouse CD51 mAb (RMV-7) | BD Biosciences | Cat #551187 |
| APC-CY7 mouse anti-mouse NK1.1 mAb (PK136) | BD Biosciences | Cat #560618 |
| Pacific Blue Hamster anti-mouse CD3e mAb (500A2) | BD Biosciences | Cat #558214 |
| NFIL3 (E4BP4), Alexa Flour® 647, Clone: 1218A | Fisher (R&D) | IC8888R100 |
| Alexa Fluor 647mouse anti-STAT4 (pY693) | BD Biosciences | Cat #558137 |
| Smad2(pS465/pS457)/Smad3(pS423/pS425) Mouse, PE, Clone: 072-670, BD | BD Biosciences | BDB562586 |
| TGF-beta RI/ALK-5, APC, Clone: 141231, 100 tests | R&D Systems | FAB5871A100 |
| Anti-Mouse CD73 eFluor 450 mAb (eBioTY/11.8) | eBioscience | Cat #48-0731-82 |
| BV605 mouse anti-mouse TIGIT mAb (1G9) | BD Biosciences | Cat #744212 |
| Anti-h/m AF488 IL12RB2 | R&D systems | Cat #FAB1959G |
| PE Anti-mouse CD200R mAb (OXr2) | BioLegend | Cat #123907 |
| Anti-mouse CD3e eFluor 450 mAb (145-2C11) | eBioscience | Cat #48-0031-82 |
| NFIL3/E4BP4 Alexa Fluor 488 | Fisher (R&D) | Cat# IC8888G |
| Pe/Cy7 anti-mouse CD127 (IL-7Ra) mAb (A7R34) | Biolegend | Cat #135014 |
| Anti-mouse CD122 e Fluor 450 mAb (TM-b1) | eBioscience | Cat #48-1-222-82 |
| FITC Hamster anti-mouse CD3e mAb (145-2C11) | BD Biosciences | Cat #553062 |
| FITC mouse anti-mouse NK1.1 mAb (PK136) | BD Biosciences | Cat #553164 |
| Anti-Hu/MO CD45R (B220) FITC mAb (RA3-6B2) | Thermo Fisher | Cat #11-0452-82 |
| PE Hamster anti-mouse CD11c mAb (HL3) | BD Bioscience | Cat #553802 |
| APC anti-mouse CD283 TLR3 mAb (11F8) | Biolegend | Cat #141906 |
| APC CY7 Anti-mouse CD8a mAb (53-6.7) | Biolegend | Cat #100714 |
| Anti-m TGFBR1/ALK5 APC | R&D systems | Cat #FAB5871A |
| PE/CY7 anti-mouse/rat CD61 mAb (HMb3-1) | Biolegend | Cat #104317 |
| APC Ly-49H anti-mouse mAb (3D10) | Thermo Fisher | Cat# 17-5886-82 |
| PE Anti Mouse CD218a IL-18Ra mAb (P3TUNYA) | eBioscience | Cat# 12-5183-82 |
| APC CY7 Rat anti-mouse CD11b mAb (M1/70) | BD Biosciences | Cat# 557657 |
| SOCS1 (A156) Antibody | Cell Signaling | Cat# 3950S |
| STREPTAVIDIN PE | BD Bioscience | Cat# 554061 |
| PE mouse anti-mouse CD212 | BD Biosciences | Cat# 551974 |
| PE Rat anti-mouse CD132 | BD Biosciences | Cat# 554457 |
| anti-CD29, FITC, Clone: Ha2/5, BD | BD Biosciences | BDB561796 |
| FITC Rat anti-mouse CD43 mAb (s7) | BD Biosciences | Cat# 553270 |
| FITC anti-mouse LY49D mAb (49-h4) | Biolegend | Cat#138605 |
| APC anti-mouse NKG2D mAb (CX5) | eBioscience | Cat# 17-5882-82 |
| PE Anti-mouse NKG2A b6 mAb (16a11) | Ebioscience | Cat# 12-5897-82 |
| E450 anti-mouse/rat KI67 mAb (SolA15) | eBioscience | Cat# 48-5698-82 |
| FITC Rat anti-mouse Ly-6G and Ly-6C GR1 mAb (RB6-8C5) | BD Bioscience | Cat# 553127 |
| FITC Rat anti-mouse TER119/Erythroid Cells mAb (TER-119) | BD Biosciences | Cat# 557915 |
| APC Rat anti-mouse CD135 FLT3 mAb (A2F10.0) | BD Biosciences | Cat# 560718 |
| PE anti-mouse CD94 mAb (18d3) | eBioscience | Cat# 12-0941-81 |
| Bacterial and Virus Strains | ||
| MCMV Smith Strain | French Lab | |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Mouse IL-3 | Peprotech | Cat# 213-13 |
| mouse IL-15 | Peprotech | Cat# 210-15 |
| Mouse IL-12 | Peprotech | Cat# 210-12 |
| Recombinant Human TGF-B1 (HEK293 derived), 10ug | Peprotech | Cat# 100-21 |
| RPMI1640 | GE Healthcare Life Science | Cat# SH30027.02 |
| Fetal Bovine Serum (FBS) | Sigma | Cat # F2442 |
| Pen Strep | GE Healthcare Life Science | Cat# SV30010 |
| NEAA | GE Healthcare Life Science | Cat# SH30027.02 |
| Sodium Pyruvate | GE Healthcare Life Science | Cat# SH30239.01 |
| L-Glutamine | Hyclone | Cat# SH30239.01 |
| HEPES | GE Healthcare Life Science | Cat# SH30237.01 |
| fixation/permeabilization kit | BD Bioscience | Cat# 51-2090KZ |
| fixation/permeabilization concentrate | Invitrogen | Cat# 00-5123-43 |
| fixation/permeabilization diluent | Invitrogen | Cat# 00-5223-56 |
| Trizol | Ambion Life Technologies | Cat# 15596026 |
| poly I:C | Invitrogen | Cat # tlrl-pic |
| 7AAD | Sigma | Cat# A9400 |
| Cell Trace Violet | Thermo Fisher | C34557 |
| Cell Trace Yellow | Thermo Fisher | C34567 |
| Phosphate Buffer Saline (PBS) | Hyclone | Cat# SH30028.03 |
| Collagenase | Sigma | Cat# C9407-100MG |
| DNase | Sigma | Cat # D4527-10KU |
| Percoll | GE Healthcare | 17-0891-01 |
| 1X HBSS | Gibco | 14025-076 |
| CFSE | Sigma | 21888-25MG-F |
| 10x PBS | Fisher Scientific | BP3991 |
| Ammonium chloride, 500G | Sigma | A9434-500G |
| Potassium Chloride, 500g, for molecular biology | Sigma | P9541-500G |
| Electron Microscopy Sci. 16% Paraformaldehyde Aq Sol., Case of 10 | Fisher | 50-980-489 |
| DMEM Media | Gibco | 11965 |
| DMSO | Sigma | D2650 |
| EDTA | Corning | 46-034-CL |
| Heparin | Fresenius Kabi USA | 401807K |
| AO/PI | Nexcelom | CS2-0106-5ML |
| Ethyl Alcohol 200 Proof | Pharmco | Cat #11100020S |
| Critical Commercial Assays | ||
| EasySep™ Mouse NK Cell Isolation Kit | Stem Cell Technologies | Catalog # 19855 |
| Direct-zol RNA Microprep | Zymo Research | R2061 |
| PE Annexin V Apoptosis Detection Kit | BD Bioscience | Catalog No. 559763 |
| Automacs NK Cell Isolation Kit | Miltenyi | |
| CellTrace™ Yellow Cell Proliferation Kit, for flow cytometry | Thermo Fisher | |
| CellTrace™ Violet Cell Proliferation Kit, for flow cytometry | Thermo Fisher | C34557 |
| Promega Dual-Glo Luciferase Assay System, 10mL | Thermo Fisher | PR-E2920 |
| Deposited Data | ||
| MicroArray | GEO | GSE85476 |
| Experimental Models: Cell Lines | ||
| Mouse: 3T3 | ATCC | |
| Human: 293T | Sands Lab | |
| Experimental Models: Organisms/Strains | ||
| Mouse: Mir142−/− | Reddy Lab | n/a |
| Mouse: Mir142f/f | Bolden Lab | n/a |
| Mouse: CD45.2 C57BL/6J | The Jackson Laboratory | Cat# JAX: 000664 |
| Mouse: CD45.1 C57BL/6J | The Jackson Laboratory | Cat# JAX: 002014 |
| Mouse: Ncr1-icre | Vivier Lab | |
| Mouse: Il15−/− | Li Lab | |
| Oligonucleotides | ||
| has-miR-142-3p, S(50RT/150 PCR rxns) | Life Technologies | 000464 |
| has-miR-142-5p, S(50RT/150 PCR rxns) | Life Technologies | 002248 |
| snoRNA135, TaqMan microRNA control Assay, S(50RT/150 PCR rxns) | Life Technologies | 001230 |
| Software and Algorithms | ||
| FlowJo | Treestar Inc | |
| Automacs | Miltenyi Biotec | |
| Cellometer | Nexcelom | |
| GraphPad Prism 8 | GraphPad | |
| Image Lab | BioRad | |