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. Author manuscript; available in PMC: 2020 Sep 1.
Published in final edited form as: Neurotoxicology. 2019 Jul 16;74:209–220. doi: 10.1016/j.neuro.2019.07.002

Figure 3 -. Time course of behavioral changes and neurodegeneration after 6-OHDA larval dosing.

Figure 3 -

– (A) Area exploration normalized to size and (B) neurodegeneration scores over the course of 3 days following 1h L1 exposure to 6-OHDA in BY200. (C; D) Area exploration normalized to size in N2 and BY200 over the course of 3 days following 1h L1 exposure to 6-OHDA. “AE/Size (% control)” shows significance in comparison to strain-matched 0mM 6-OHDA control. “AE/Size” shows significance in comparison to dose-matched N2 control. All experiments were completed with two biological replicates. (A) n=777; 2-way ANOVA 6-OHDA*Age p=0.0006; Tukey HSD [10mM,3d vs. 0mM,3d p=0.0015]. (B) n=908 neurons/227 worms; 2-way ANOVA 6- OHDA*Age p<0.0001; Tukey HSD [15mM,3d vs. 0mM,3d p<0.0001; 15mM,2d vs. 0mM,2d p<0.0001; 10mM,2d vs. 0mM,2d p<0.0001; 15mM,1d vs. 0mM,1d p<0.0001; 10mM,1d vs. 0mM,1d p=0.0021; 10mM,3d vs. 0mM,3d p=0.0222]. (C) n=1489 3-way ANOVA 6-OHDA *Age*Strain p<0.0001; Tukey HSD [BY200,10mM,3d vs. BY200,0mM,3d p=0.0011]. (D) n=1489 3-way ANOVA 6- OHDA*Age*Strain p<0.0001; Student t-test [N2,0mM,3d vs. BY200,0mM,3d p=0.0003; N2,15mM,3d vs. BY200,15mM,3d p=0.0043].