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. Author manuscript; available in PMC: 2020 Sep 17.
Published in final edited form as: Immunity. 2019 Aug 27;51(3):508–521.e6. doi: 10.1016/j.immuni.2019.05.021

Figure 7. IL-1β signaling in intestinal epithelial cells is required for LCWE-induced KD vasculitis.

Figure 7.

(A) Scheme for the generation of Vil1CreIl1r1Δ/Δ mice. (B) Immunofluorescence analysis showing absence of IL-1R1 expression (red) in SI sections of Vil1CreIl1r1Δ/Δ as compared with Il1r1fl/fl mice. (C) FITC-dextran intestinal permeability assay in PBS-injected Il1r1fl/fl, LCWE-injected Il1r1fl/fl and LCWE-injected Vil1CreIl1r1Δ/Δ 24 h post-injection (n=7–10 per group). Data normalized to PBS controls. (D, E) Representative H&E staining of heart sections (D) and heart vessels inflammation score (E) of LCWE-injected Il1r1fl/fl and Vil1CreIl1r1Δ/Δ (n=14 per group). Scale bars: 500μm. (F) Representative pictures of abdominal area and H&E staining of abdominal aorta cross-section from LCWE-injected Il1r1fl/fl and Vil1CreIl1r1Δ/Δ. Scale bars: 200μm. (G) Measurement of maximal abdominal diameter of LCWE-injected Il1rl1fl/fl and Vil1CreIl1r1Δ/Δ (n=12–14 per group). (H) Representative H&E sections and immunostaining of CD31 (green) and IgA (red) in the heart coronary artery of LCWE-injected Il1r1fl/fl (1; non-obstructed and 2; obstructed coronary) and Vil1CreIl1r1Δ/Δ. Scale bars: 25μm. (I) H&E abdominal aorta cross sections staining and immunostaining of CD31 (green) and IgA (red) in the abdominal aorta of LCWE-injected Il1r1fl/fl. Scale bars: 10μm. (J) IgA immunofluorescence quantification in the heart and abdominal aorta of LCWE-injected Il1rl1fl/fl and Vil1CreIl1r1Δ/Δ (n=5 per group). Data are presented as mean ± s.e.m. *p<0.05,**p<0.01, ***p<0.001 by 1-way ANOVA with Bonferroni post-test (C) or two-tailed unpaired t test (E, G and J) and pooled from 2 to 4 independent experiments.