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. Author manuscript; available in PMC: 2020 Nov 1.
Published in final edited form as: Biochim Biophys Acta Mol Basis Dis. 2019 Jul 27;1865(11):165516. doi: 10.1016/j.bbadis.2019.07.010

Figure 3. Role of active Perk in eIF2α phosphorylation in GalT-deficient mouse fibroblasts.

Figure 3.

a) Quantification of phospho-eIF2α and its downstream targets Atf4 and Chop in GalT−/− fibroblasts in the presence/absence of 1 μM GSK2606414 or 2 μg/ml tunicamycin. (Representative image from three independent experiments using three different cell lines that yielded identical results.)

b) Expression level of Atf4 in GalT−/− fibroblasts in the presence/absence of 50 μM Salubrinal or 300 nM ISRIB.

c) Protein expression levels of Atf4 in WT and GalT−/− mice fibroblasts (n=3 in each group).