FIG 2.

The Cek1 MAPK is hyperphosphorylated in lrg1ΔΔ cells compared to wild type. (A) Proteins were isolated from Candida cells in log phase. Western blotting was performed with anti-phospho-p44/42 antibody (stains phosphorylated Mkc1 and Cek1), as well as anti-Mkc1 and-Cek1 antibodies (stain total Mkc1 and Cek1 proteins, respectively). (B) The phospho-Mkc1 bands were quantified and normalized based on the total Mkc1 bands and tubulin. (C) The phospho-Cek1 bands were quantified and normalized based on the total Cek1 bands and tubulin. The graphs in both cases were based on quantification of 3 blots, and statistical analysis was performed using one-way ANOVA. ****, P < 0.0001; ns, not significant.