FIG 6.
Hyperactivation of Cek1 leads to attenuated fungal virulence in a mouse model of systemic infection. C. albicans strains that were wild type (DAY286) or carried the PtetOFF-STE11ΔN467 allele were cultured overnight at 30°C with doxycycline to repress Ste11ΔN467 expression. The overnight cultures were diluted to 106 cells/ml, and 100-μl volumes of the suspensions were injected into the lateral tail veins of outbred ICR mice. Mice were given 5% sucrose in their drinking water either without doxycycline or with 2 mg/ml of doxycycline to repress Ste11ΔN467 expression in vivo. The symptoms of illness were monitored over 21 days. Each group had 10 mice. *, P = 0.016.