FIG 1.

SigN is required for loss of cell viability after MMC treatment. (A) Optical density (OD₆₀₀) growth curve of wild type (open circles, DK607) and sigN mutant (closed circles, DK3287). The x axis is the time of spectrophotometry after MMC addition. (B) CFU growth curve of wild type (open circles, DK607) and sigN mutant (closed circles, DK3287). The x axis is the time of dilution plating after MMC addition. (C) Western blot analysis of wild-type DK607 cell lysates harvested at the indicated time after MMC addition and probed with either anti-SigN antibody or anti-SigA antibody. On the right is a single panel of the same strain for comparison 2 h after mock MMC addition.