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LexA binds to the PsigNUP promoter region. Electrophoretic mobility shift assays were performed with radiolabeled DNA of PrecA (A), PsigNUP (B), PsigNDN (C), and PsigNUP* (D) mutated for the putative LexA binding site. Purified LexA protein was added to each reaction mixture at the indicated concentration.
