Phototropic Response of Arabidopsis Inflorescence Stems under Unilateral UV-B Irradiation.
(A) Photographs of Arabidopsis inflorescence stems having a height of ∼5 cm that were exposed to unilateral 1.3 µmol m−2 s−1 311 nm irradiation for 24 h (UV-B treatment). This UV-B treatment was given to four different genotypes: the wild type (Col), lack-of-function UVR8 mutant uvr8-6, phototropin double mutant phot1 phot2, and the triple mutant phot1 phot2 amiUVR8. The direction of the UV-B irradiation is indicated by white arrows.
(B) and (C) Kinetic analysis of the phototropic bending response of the inflorescence stems upon UV-B treatment. The bending angle was quantified every 15 min for 8 h. UVR8-deficient lines (uvr8-6 and uvr8-7), phototropin double mutant with or without UVR8 (phot1 phot2 and phot1 phot2 amiUVR8, respectively), hy5 and hyh single and double (hy5 hyh) mutants, and a quadruple mutant (phot1 phot2 hy5 hyh) are compared with the corresponding wild type (Col and Ws). Error bars indicate se (n ≥ 8).