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. 2019 Jul 2;31(9):2052–2069. doi: 10.1105/tpc.18.00786

Figure 5.

Figure 5.

RRC1 Is Involved in Light Regulation of Pre-mRNA Splicing.

(A) and (B) Scatterplots indicate the splicing efficiency changes for 774 light-regulated splicing events (the splicing events with the Delta_PSI/PIR > 3%, FDR < 0.5 while comparing Col-0 dark and Rc-irradiated samples) in Col-0 wild-type (A) and rrc1-3 (B) mutant backgrounds. The x axis is the splicing efficiency (folded root of PSI/PIR) in the dark, and the y axis is the splicing efficiency (folded root of PSI/PIR) after light treatment.

(C) Overlay of (A) and (B). The pair-wise t test indicates the Delta_PSI/PIR of the Col-0 wild type (Delta_PSI/PIR= Col-0_PSI/PIR_D – Col-0_PSI/PIR_Rc) is significantly different from the Delta_PSI/PIR of rrc1 mutant (Delta_PSI/PIR= rrc1-3_PSI/PIR_D – rrc1-3_PSI/PIR_Rc), P < 0.01.

(D) Heatmaps of the quantified splicing profiles of 774 light-regulated splicing events. Delta_PSI/PIR (Delta_PSI/PIR= PSI/PIR_D − PSI/PIR_Rc) values were used to quantify the splicing efficiency changes in response to light. The events are ranked by the value of Delta_PSI/PIR in Col-0 background. Blue color indicates low Z-scores, whereas red color indicates high Z-scores. Red light intensity used was at 7 μmol m−2 s−1.