Fig. 6.

Lag3 knockout reduced the antigen-specific suppression of OVA DNTs. Relative Lag3 mRNA expression in OVA DNTs and CD4⁺ T cells was measured by a real-time PCR and b flow cytometry. Mice received WT OVA DNTs or Lag3^−/− OVA DNTs by intravenous adoptive transfer to treat OVA-induced airway inflammation. c Lung sections were stained with H&E to measure the accumulation of infiltrating inflammatory cells (Scale bars, 100 μm). d Lung eosinophils (CD11b⁺Siglec F⁺CD11c^-), e DCs (CD11c⁺MHC-II⁺), CD11b⁺ DCs (CD11b⁺CD11c⁺MHC-II⁺) and f Tfh cells (CD4⁺B220^-CXCR5⁺PD-1⁺) were assessed by flow cytometry. g GzmB expression in WT DNTs and Lag3^−/− DNTs were measured by flow cytometry. h Relative Prf1 and Fasl mRNA expression levels in WT DNTs and Lag3^−/− DNTs were measured by real-time PCR. i The apoptosis of DNT cells was detected by flow cytometry. j The expression of CD69 and Ki67 were detected by flow cytometry. Data are shown as the mean ± SEM; n = 4–5 mice per group. One-way ANOVA and Student’s t-test were used to calculate significance. *P < 0.05; **P < 0.01; ***P < 0.001. Source data are provided as a source data file