Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Sep 18;10:4241. doi: 10.1038/s41467-019-12017-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — Inflammatory response induced in the lungs during PbAluc-infection in C57BL/6 mice. a Relative percentage of the mean counts of each immune-cell population found in the lungs of naive (n = 9) and PbAluc-infected mice (n = 11) at 7 dpi derived from the total number of cells shown in Supplementary Fig. 1. MHCII^- AM MHC-Class II negative alveolar macrophages, MDM monocyte-derived macrophages, DC dendritic cell, cDC conventional dendritic cell, pDC plasmacytoid DC, NK natural killer. b Total number of CD4⁺LFA-1⁺ and CD8⁺LFA-1⁺ T cells and c Pb1-specific CD8⁺T cells accumulated in the lungs of naive (n = 9) and infected mice (n = 7). d Tetramer and intracellular cytokine staining was performed on lungs sequestered leukocytes from naive (n = 3) and PbAluc-infected mice (n = 6). Cells were incubated with Brefeldin for 3 h without re-stimulation. The indicated dot plot (top) was used to analyze the Pb1-specific CD8⁺T cells and to gate the IFN-γ⁺ and GrzB⁺ population. The total number of Pb1-specific CD8⁺T cells secreting each cytokine is represented graphically (bottom). The data shown are representative of two independent experiments. The data represent the mean ± SD; *p < 0.05, **p < 0.01, ***p < 0.001 by Mann–Whitney test (b–d)