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. 2019 Sep 18;10:4241. doi: 10.1038/s41467-019-12017-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Lung endothelial cell cross-presentation of PbAluc antigen to Pb1-specific CD8⁺T cells during PbAluc infection. Lung microvessels were isolated from naive (n = 6) and PbAluc-infected C57BL/6 5 (n = 6), 6 (n = 5) and 7 dpi (n = 5), and sorted into two populations: CD45⁺CD31⁻ leukocytes and CD45^-CD31⁺ endothelial cells. Each sorted population was tested for Pb1 cross-presentation by incubating with LR-BSL8.4a cells and staining with X-gal. The spot count was normalized per 50,000 cells and is represented on a log scale. The data represent the mean; **p < 0.01, ****p < 0.0001, ^ statistically significant compared to naive mice (p < 0.05) by ANOVA with Bonferroni’s post-test