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. 2019 Sep 18;9:13510. doi: 10.1038/s41598-019-49938-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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LTP inhibits mast cell activation. (a) Mast cells were generated from mouse bone-marrow derived lineage negative cells by culture with mIL-3 and mSCF for 8 weeks. The percentage of mast cells exceed 94%. (b) A schematic diagram showing the generation of LTP. Non-thermal plasma was treated for 60 sec per ml and used for in vitro experiments. (c) All Western blotting experiments were performed under the same condition. LTP treatment inhibits NF-κB activation in PMA/A23187-stimulated mast cells. LTP treatment inhibits (d) TNF-α, (e) IL-6, and (f) IL-13 expression in PMA/A23187-stimulated mast cells. LTP treatment reduced the secretion of (g) TNF-α, (h) IL-6, and (i) IL-13 by activated mast cells. *P < 0.05, **P < 0.01, ***P < 0.001.