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. 2019 Sep 18;9:13510. doi: 10.1038/s41598-019-49938-9

Figure 6.

Figure 6

LTP treatment inhibits allergic inflammatory responses in HaCaT. (a) All Western blotting experiments were performed under the same condition. LTP treatment inhibited IL-4-induced STAT6 activation in HaCaT. IL-4 was stimulated with or without LTP for 15 min and the cells were collected for p-STAT6 detection using western blot analysis. The level of p-STAT6 was measured using ImageJ software. (b) LTP treatment inhibited IL-4-induced CCL26 expression in HaCaT. (c) All Western blotting experiments were performed under the same condition. LTP treatment inhibited TNF-α/IFN-γ-induced NF-κB activation in HaCaT. HaCaT cells were stimulated with TNF-α and IFN-γ for 15 min and the cells were harvested for p-NF-κB level using western blot analysis. The level of p-NF-κB was measured using ImageJ software. The expression of pro-inflammatory chemokine, (d) IL-8, and cytokine, (e) IL-6 and (f) TNF-α was detected in TNF-α/IFN-γ-stimulated HaCaT with or without LTP treatment using real-time PCR analysis. *P < 0.05, **P < 0.01, ***P < 0.001.