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. 2019 Sep 18;10(10):688. doi: 10.1038/s41419-019-1927-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Detection of the mRNA expression levels of p21, Gadd45α, p27, cyclin B1, PTEN, p53, E2F1, and Wee1 in control and CELF6 knockout HCT116 cells. b The protein levels of p53, p21, p27, Wee1, cyclin B1, Gadd45α, and CELF6 were analyzed by immunoblotting. c Quantitative RT-PCR and western blot analysis in HCT116 cells transfected with control or CELF6 siRNA. d HCT116 were transfected with pcDNA3.1 or His-CELF6 plasmids for 48 h, qPCR was performed to determine the expression of p21, Gadd45α, p27, cyclin B1, PTEN, p53, E2F1, CELF6, and Wee1. e HCT116 cells overexpressing His-CELF6 or GFP-CELF6 were subjected to immunoblotting analysis to determine the protein expression of p53, p21, p27, Wee1, cyclin B1, Gadd45α, and exogenous CELF6