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. 2019 Sep 18;10(10):688. doi: 10.1038/s41419-019-1927-0

Fig. 7. CELF6 modulates cell cycle progression and cell proliferation.

Fig. 7

a HCT116 cells transfected with control, p21, or CELF6 siRNA for 48 h, the cell cycle distribution was analyzed by flow cytometry. b Control or CELF6 knockout HCT116 cells were fixed and stained with PI, the cell cycle distribution was analyzed by flow cytometry. c HCT116 cells overexpressing empty vector or His-CELF6 were fixed, the percentage of G0/G1, S, and G2 phase cells was determined by flow cytometry. d HCT116 p53−/− or e HCT116 p21−/− cells were transfected with control or CELF6 siRNA, pcDNA3.1, or His-CELF6 for 48 h, the cell cycle distribution was analyzed by flow cytometry. f Control or CELF6 knockout cells were labeled with EdU for 30 min, cells were fixed and performed Click-iT EdU Alexa Fluor 555 imaging assay. The percentage of EdU-positive cells was determined by counting at least 300 cells for each sample. Scale bar, 50 μm. g Colony formation assay in control and CELF6 knockout HCT116 cells. h CCK8 cell proliferation assay in HCT116 cells transfected with control siRNA, CELF6 siRNA, or CELF6 siRNA plus p21 plasmid. i CCK8 cell proliferation assay in HCT116 p21−/− or j HCT116 p53−/− cells transfected with control or CELF6 siRNA, pcDNA3.1, or His-CELF6