Fig. 6. P38 MAPK signaling pathway serves as a negative regulator in dynasore induced proliferative inhibition.

a Western blot analysis of p-p38 and p38 of OS cells after treated with dynasore and/or cisplatin. The relative protein expression of p-p38 and p38 in MNNG/HOS (b) and MG-63 (c) were shown as histogram. d The cell viability of OS cells after treated with SB239063 (10 μM) followed by dynasore exposure. After suppression of p38 MAPK pathway by SB239063, cell proliferations were analyzed by EdU staining (MNNG/HOS (e, g) and MG-63 cells (f, h)) and cloning formation assay (I-K). *p < 0.05 and **p < 0.01 vs. DMSO group. SBD, co-treated with 50 μM dynasore and 10 μM SB239063 as described in “Materials and methods” section. Scale bar: 50 μm