Figure 3.

Complementation of the Atvip2 mutant by constitutive expression of AtVIP2. Three independent transgenic events were generated, and molecularly characterized before performing root transformation assays. (a) Roots of the wild-type, vip2 mutant and three transgenic Atvip2 lines expressing AtVIP2 cDNA were infected with a tumorigenic strain A. tumefaciens A208, at 1 × 10⁷ CFU/ml concentration. Tumors incited on the roots were visualized and scored 4 weeks after infection. (b) Stable GUS expression. Roots of the wild-type, Atvip2 mutants and transgenic Atvip2 lines expressing AtVIP2 cDNA were inoculated with A. tumefaciens strain GV3101 carrying the uidA-intron gene within the T-DNA at 1 × 10⁷ CFU/ml concentration. The inoculated roots were stained with X-Gluc 2-3 weeks post infection. All the experiments were repeated two times with similar results.