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Analysis of ScFv-PEG2000-DSPE by MALDI-TOF–MS. a The coupling schematic of PEG2000-DSPE and ScFv-Cys. b The purified product of DSPE-PEG2000-ScFv was verified by MALDI-TOF MS. The theoretical molecular weight (MW) of malDSPE-PEG2000 is 3 kDa, so the experimental MW of ScFv and DSPE-PEG2000-ScFv were exactly 26 kDa and 29 kDa, respectively
