Figure 5.

Inhibition of glycolytic metabolism abolishes the functionality of CD8+ T cells. Purified CD8+ T cells were stimulated with αCD3 antibody (5 μg/mL) or/and R848 (10 μg/mL) for 24 h in the presence of 2DG (2 mM) or in the glucose-free medium supplemented with pyruvate. (A) The activation of CD8+ T cells was assessed by staining with an αCD44 antibody. (B) IFN-γ secretion in CD8+ T cells was determined by a specific ELISA. (C) T-bet and Eomes expression in CD8+ T cells was measured by flow cytometry and presented as MFI. (D) IRF4 expression in CD8+ T cells was assessed by flow cytometry. Data are representative of three independent experiments. All data are presented as mean ± SD. (*p < 0.05; ***p < 0.001; ns, not significant) Statistical relevance was determined by Two-way ANOVA.