FIGURE 5.

Late Endocytic Mediators of GRP78 and DMP1 Internalization. hPDLSCs were subjected to osteogenic differentiation or control media for 2 days prior to treatment with rDMP1. Both groups were grown to 70–80% confluency and serum starved for 4 h prior to treatment with rDMP1. (A) Representative confocal images showing localization of Rab7 (FITC-Green) and GRP78 (TRITC Red). (B) Localization of Rab11 (FITC-Green) and GRP78 (TRITC Red). Images were acquired with a Zeiss Meta 710 Confocal Microscope. Arrows denote areas of colocalization between GRP78 and Rab7 or 11. Scale bar = 10 μm.