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. 2019 Sep 12;10:1175. doi: 10.3389/fphys.2019.01175

FIGURE 7.

FIGURE 7

Protein and Gene Expression Analysis of GRP78, DMP1 and its Endocytic Regulators. (A) Total proteins were isolated from PDLSCs (control); PDLSCs grown under osteogenic differentiation conditions; PDLSCs stimulated with DMP1 for 24 h; PDLSCs stimulated with DMP1 for 24 h and grown under osteogenic differentiation conditions for 2 days. Western blots analysis was performed with antibodies against Cav1, DMP1, Dynamin, GRP78, Rab5, and Rab7. Equal loading of the proteins was confirmed by loading with Tubulin. (B) Total RNA was isolated from hPDLSCs in normal growth conditions and two-day osteogenic differentiation conditions and subjected to quantitative real-time PCR. The results were normalized to one. Fold change was determined with respect to the control which was normalized as 1. Values are the mean ± standard deviation of triplicate samples. A statistically significant difference is denoted with ∗P < 0.05 vs. control. Comparisons were performed using the Student’s t-Test.