FIGURE 8.

Differentiation of hPDLSCs cultured in Control and Osteogenic Conditions. (A) hPDLSCs were grown for 0, 7, and 14 days in control or osteogenic differentiation conditions prior to total protein extraction. Western Blots were developed with antibodies against DPP, GRP78, Col1a1, and Tubulin as a loading control. (B) hPDLSCs were grown for 0, 7, and 14 days in control or osteogenic differentiation conditions prior to total RNA extraction. qPCR was performed with osteogenic gene markers. The results were normalized to hGAPDH. Fold change was determined with respect to the control which was normalized as 1. (C) hPDLSCs were grown for 0, 7, and 14 days in control or osteogenic differentiation conditions (OD) prior to total protein extraction. Western Blots were developed with antibodies against full length DMP1. Values are the mean ± standard deviation of triplicate samples. A statistically significant difference is denoted with ^∗P < 0.05 vs. control. Comparisons were performed using the Student’s t-Test.