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. 2019 Sep 12;7:196. doi: 10.3389/fcell.2019.00196

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Copyright © 2019 Ren, Sun, Clinton and Yang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Identification and characterization of chicken embryo transcripts. (A) Raw Iso-Seq reads were divided into five groups to filter FLNC reads. (B) FLNC reads were divided into five groups based on their genome mapping characteristics to filter transcripts. (C) All transcripts were divided into three groups based on their genome annotations. (D) Density plot of the length of the PacBio transcripts and previously annotated transcripts. (E) Distribution of the number of transcripts per gene generated by PacBio data and reference genome data. (F) Comparison of exon numbers among genes producing different numbers of transcripts. (G) Comparison of gene length among genes producing different numbers of transcripts. (H) Identified genes and transcripts in embryos at different developmental stages by Iso-Seq.