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. 2019 Sep 12;10:2170. doi: 10.3389/fimmu.2019.02170

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Copyright © 2019 Dekkema, Bijma, Jellema, Van Den Berg, Kroesen, Stegeman, Heeringa, Abdulahad and Sanders.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Overexpression of miR-142-3p reduces the suppressive capacity of _MTregs in vitro. _MTregs were transfected with scrambled (SCR) or miR-142-3p mimic (MIM-142-3p). Live _MTregs were sorted and cocultured with responder T (T_RESP) cells; after 48 h of stimulation, T_RESP cell proliferation was determined (A,B). Transfection with MIM-142-3p significantly increased miR-142-3p levels (C) and reduced the suppressive capacity of _MTregs (D). miR-142-3p levels correlated negatively with the suppressive capacity (E). Transfection with MIM-142-3p further decreased ADCY9 mRNA levels (F) and correlated negatively with miR-142-3p levels (G). Additionally, cAMP levels were significantly lower in MIM-142-3p-transfected _MTregs (red) than in SCR-transfected _MTregs (black) (H). *p = 0.05–0.01.