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. 2019 Sep 12;10:2170. doi: 10.3389/fimmu.2019.02170

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Copyright © 2019 Dekkema, Bijma, Jellema, Van Den Berg, Kroesen, Stegeman, Heeringa, Abdulahad and Sanders.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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cAMP elevating agent Forskolin increases Treg function via the increase of cAMP. As proof of principle we tried to restore Treg function after miR-142-3p overexpression using cAMP elevating agent Forskolin. _MTregs were transfected with scrambled (SCR) or miR-142-3p mimic (MIM-142-3p). Live _MTregs were sorted and cocultured with responder T (T_RESP) cells; after 48 h of stimulation, T_RESP cell proliferation was determined (A). Upon treatment with Forskolin, cAMP levels increased significantly (B). Moreover, forskolin treatment tended to restore suppressive function in miR-142-3p overexpressed _MTregs (C).*p = 0.05–0.01, **p = 0.01–0.001.