Fig. 3. Knockdown of SNHG3 inhibited metastasis of GC cells both in vitro and in vivo.

a, b Knockdown of SNHG3 significantly reduced, and overexpression of SNHG3 increased the migratory ability of GC cells (Wound healing assay). **P < 0.01. c, d Knockdown of SNHG3 significantly reduced, and overexpression of SNHG3 increased the invasive ability of GC cells (Transwell assay). **P < 0.01. e, f H&E staining of the metastatic nodules in the lung of MKN-45 cells which stably transfected with SNHG3 shRNAs (sh-SNHG3-1 and sh- SNHG3-2) or empty vector (sh-CTR) following tail vein injection into nude mice (×200 scale bars) and incidence of lung metastasis in mice following tail vein injection of the respective MKN-45 cells. *P < 0.05; **P < 0.01. χ² test for f, student’s t-test for others