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Biotinylated miR-373 can regulate the expression of E-cadherin. (A) The identified miR-373 binding site in E-cadherin promoter. MCF-7 cells were transfected with miR-373, NC miRNA, or bio-miR-373 for 48 h. Then semi-quantitative PCR (B), real-time PCR (C), and Western blotting (D) were performed to detect the expression of E-cadherin. n = 3, **P < 0.01 compared with the sample transfected with NC miRNA.
