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. 2019 Sep 12;10:778. doi: 10.3389/fgene.2019.00778

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Copyright © 2019 Xun, Tang, Hu, Xiao, Long, Gong, Wei, Wei and Xiang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Promoters of E-cadherin and CSDC2 can be pulled down by biotinylated miR-373. Bio-miR-373 or bio-NC-miRNA was transfected into MCF-7 cells for 24 h. Then cells were harvested, and the DNA fragments were enriched via DNA–miRNA pull-down assay. (A) The enrichments of E-cadherin promoter were measured by semi-quantitative PCR. (B) The enrichments of CSDC2 promoter were measured by semi-quantitative PCR. (C) Real-time PCR was performed to detect the enrichments of E-cadherin and CSDC2 promoters. U6 fragments added in the samples were taken as an endogenous control. n = 3, **P < 0.01 compared with the sample transfected with bio-NC-miRNA.