Skip to main content
. 2019 Sep 12;10:778. doi: 10.3389/fgene.2019.00778

Figure 5.

Figure 5

The regulation of identified genes by miR-373 in HeLa cells. (A) HeLa cells were transfected with miR-373 or NC-miRNA. Cells were harvested after 24, 48, 72, or 96 h post-transfection. Real-time PCR was used to measure the mRNA level of ALOX5, ARID2, E-cadherin, KIAA1958, PDE4D, SUN1, ZNF76, and ZNF385B genes. The expression of β-actin was used as the internal reference. n = 3, *P < 0.05, **P < 0.01 compared with the sample transfected with NC miRNA. (B) Bio-miR-373 or bio-NC-miRNA was transfected into HeLa cells. After 24 h post-transfection, DNA–miRNA pull-down assays were performed to enrich the miR-373 binding sequences. Then real-time PCR was performed to detect the enrichments of the genomic sequences in ALOX5, ARID2, E-cadherin, KIAA1958, PDE4D, SUN1, ZNF76, and ZNF385B genes. U6 fragments added in the samples were taken as an endogenous control.