Figure 7.

The identified genes via next-generation sequencing can be regulated by miR-373. (A) Bio-miR-373 or bio-NC-miRNA was transfected into MCF-7 cells for 24 h. Then cells were harvested, and the DNA fragments were enriched via DNA–miRNA pull-down assay. The enrichments of TTC34, EVI5, TPM1, RPL37, KIAA1377, and FANCC promoters were measured by semi-quantitative PCR. (B) MCF-7 cells were transfected with miR-373 or NC-miRNA. Cells were harvested after 24, 48, or 72 h post-transfection. Real-time PCR was used to measure the mRNA level of identified genes. The expression of β-actin was used as the internal reference. n = 3, *P < 0.05, **P < 0.01 compared with the sample transfected with NC miRNA.