FIG. 3.

Histologic and subcellular location of perivascular antigens. (Left) Venn diagram to demonstrate the location of n = 328 perivascular antigens within different layers of the vessel wall, including the tunica intima, media, and adventitia. The majority of antigens were found within the tunica intima only (n = 155) (A). Next most common, n = 96 antigens were specific to the tunica media. n = 69 antigens showed shared immunostaining between both the tunica intima and the media. (Right) All antigens expressed in the tunica intima (n = 229) were next segregated by known subcellular location. The majority of proteins were restricted to the intracellular compartment (n = 147) (B) In total, n = 60 proteins with known membranous location were identified, including n = 32 proteins with expression restricted to the membrane. These 60 membranous proteins within the tunica intima were next used for cross-referencing with the transcriptome of human pericytes.