Table 3.
Perivascular RNA Transcripts Expressed Among Freshly Sorted but Not Culture Expanded CD146+ Human Pericytes
| Gene | Freshly sorted pericytes (RPKM) | Cultured pericytes (RPKM) |
|---|---|---|
| HLA-DRA | 401 | 1 |
| AKAP1 | 211.25 | 1 |
| CD93 | 126 | 11 |
| TMEM135 | 115 | 1 |
| GPR4 | 104 | 1 |
| C1orf27 | 95.5 | 1 |
| LRRN4CL | 95 | 1 |
| GFRA3 | 45 | 1 |
| ST3GAL5 | 30 | 1 |
| RNF112 | 17 | 1 |
| TMPRSS9 | 8 | 1 |
| RANBP17 | 5 | 1 |
| SLC22A15 | 1 | 1 |
| C12orf76 | 1 | 1 |
| FAM171A1 | 1 | 1 |
| ALG12 | 1 | 1 |
| TMC7 | 1 | 1 |
| ABCB7 | 1 | 1 |
| RDH16 | 1 | 1 |
| CDCP1 | 1 | 1 |
Of the 25 genes/proteins expressed present in human pericytes by both TMA and RNA-Seq, 11 genes were expressed among freshly sorted pericytes, but they were lost after culture propagation. Data were normalized by per Million mapped reads (RPKM) dataset. Corresponding heatmap is shown in Fig. 4.