Fig. 2.

Effect of catalpol on the viability of bEECs

(a) Cells were cultured in 96-well cell plates at a density of 1×10⁵ cells/mL for 24 h, and then catalpol was added at five concentrations (1 mmol/L, 0.1 mmol/L, 0.01 mmol/L, 1 μmol/L, or 0.1 μmol/L), followed by incubation for 6, 12, and 24 h. After CCK-8 was added for 2 h, the optical density was measured at a wavelength of 450 nm (OD₄₅₀). (b) Catalpol at five concentrations was added to bEEC cultures for 12 h. CCK-8 was then added and after 2 h, OD₄₅₀ was measured. The results were calculated using the following formula: cell viability (%)=(OD_treatment–OD_blank)/(OD_control–OD_blank)×100%. Data represent the mean±standard error of mean (SEM) of triplicate experiments. CG: control group; bEEC: bovine endometrial epithelial cell; CCK-8: Cell Counting Kit-8