Fig. 4.

HOTTIP promotes neutrophils induced-suppression on T cell proliferation by enhancing IL-6 expression. a Western blot analysis of STAT3 and PD-L1 proteins as well as the extent of STAT3 phosphorylation in SKOV3 cells overexpressing HOTTIP with or without IL-6 silencing. b The ability of proliferation of CFSE-labeled T cells co-cultured with neutrophils added with SKOV3 cells overexpressing HOTTIP with or without IL-6 silencing, as determined by flow cytometry. ^* p < 0.05 vs. the cells without treatment. The measurement data were summarized as mean ± standard deviation. The one-way analysis of variance was adopted for comparison among multiple groups, followed by Tukey’s post hoc test. The experiment was repeated 3 times independently