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Primer sequences for RT-qPCR
| Primer sequence | Forward (5′ - 3′) | Reverse (5′ - 3′) |
|---|---|---|
| HOTTIP | CCTAAAGCCACGCTTCTTTG | TGCAGGCTGGAGATCCTACT |
| GAPDH | ACAACAGCCTCAAGATCATCAG | GGTCCACCACTGACACGTTG |
RT-qPCR reverse transcription quantitative polymerase chain reaction, HOTTIP HOXA transcript at the distal tip, GAPDH glyceraldehyde-3-phosphate dehydrogenase
