Fig. 3.

Cluster of differentiation 68 (CD68) expression in ionized calcium binding adapter molecule 1 (Iba1)-positive microglial cells. (A) Paraffin-embedded brain sections were immunostained with Iba1 (green) for microglial cells and CD68 (red) for phagocytic cells, and subsequently counter-stained with 4, 6-diamidino-2-phenylindole (DAPI) (blue) for cell nuclei at 2 weeks after reperfusion. Both reactive and phagocytic microglial cells are shown in the inset images. 1, infarct core; 2, peri-infarct core; 3, contralateral hemisphere. Representative images were obtained from animal R326. Scale bar represents 10 μm. (B~D) Quantification of CD68- and Iba1-positive cells. The number of CD68-specific (B), Iba1-specific (C), and CD68⁺/Iba1⁺-co-expressing (D) cells was quantified using Image J cell counter. (E) The ratio of CD68⁺/Iba1⁺ cells over total Iba1⁺ cells is shown. Data are presented as mean±SEM. Each line chart symbol represents 50 regions of interest, with 2 animals in each group. (F) Linear regression analysis of the intensities of CD68- and Iba1-positive cells in the ipsilateral hemisphere from subacute and chronic groups.