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. 2019 Sep 18;7:256. doi: 10.1186/s40425-019-0736-4

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — a-c PNAd and CD31(endothelial cells) in a control muscle without pathological change. PNAd is not visible. d-f PNAd in Case 3 showing positivity (green) in the interstitial tissue, particularly on the blood vessels (red). g-i PNAd in Case 2. The merged image demonstrates a vessel strongly positive for PNAd (green) on the endothelial cells (red). j-l CCL2 in normal muscle. No positivity was detected. m-o CCL21in Case 1. The merged image shows positive CCL21 (green) on some blood vessels (red) in the perivascular space and around the muscle fibers. p-r CCL21 in Case 3. Blood vessels (CD31 red) around a muscle fiber show positivity for CCL21