TABLE 2.
Sequences of primers and probes
| Gene | Primer or probe | Sequence (5′→3′) | Tma (°C) | Product size (bp) |
|---|---|---|---|---|
| Last gene of spoVA2mob operon | spoVA2mob-F | AACCACTAGCCACGATTG | 59 | 169 |
| spoVA2mob-R | AAGGGTCTTTCTTGTGGG | 59 | ||
| spoVA2mob-probe | /FAMb /ACGAAGTCGGGCTTGGCTACA/ | 68 | ||
| gyrB | gyrB-F | ATCGTCGACAACAGTATTG | 57 | 205 |
| gyrB-R | CTTTATATCCGCTTCCGTC | 57 | ||
| gyrB-probe | /HEXc /CCCTGCGGTTGAAGTCATCATGA/ | 66 | ||
| Front part of spoVA2mob operon | spo-F | AAGGTCGAGCAAAGACTG | 59 | 1,516 |
| spo-R | ACCTGTAGCCACAACTAAC | 59 |
a
Tm, melting temperature.
b
FAM, 6-carboxyfluorescein.
c
HEX, reporter dye.