TABLE 1.
LLMDA limit of detection and reproducibility in spiked mosquito pools
| Virus(es) | PFU/ml | LLMDA detection | Log CL ratio | No. of probes positive/total | Mosquito species |
|---|---|---|---|---|---|
| DENV-2 | 102 | Negative | A. aegypti | ||
| 102 | Negative | A. aegypti | |||
| 103 | Positive | 56.7 | 20/27 | A. aegypti | |
| 103 | Positive | 60.7 | 23/33 | A. aegypti | |
| DENV-2 + MAYV | 104, 104 | Positive | 197.1, 78.5 | 46/47, 20/25 | A. aegypti |
| 105, 104 | Positive | 224.6, 122.3 | 53/54, 25/25 | A. aegypti | |
| RVFV | 104 | Positive | 52.8 | 16/19 | C. quinquefasciatus |
| ZIKV | 104 | Negative | 0 | 3/27 | A. aegypti |
| 102 | Negative | 0 | 3/27 | A. aegypti |