TABLE 3.
LLMDA and conventional PCR detection of field-collected samples
| Locality | Mosquito species | Sample size | Virus | LLMDA detectiona | PCR detectiona | % identity by Sanger sequencing (accession no.) |
|---|---|---|---|---|---|---|
| LRGV | A. aegypti | 96 | CFAV | 2 (9) | 3 (9) | 97.7 (GQ165810) |
| San Antonio | A. aegypti | 33 | CFAV | 1 (2) | 2 (2) | 100 (KJ476731) |
| Colony | A. aegypti | 40 | CFAV | 0 (4) | 0 (4) | |
| LRGV | A. albopictus | 4 | CFAV | 0 (3) | 0 (3) | |
| San Antonio | A. albopictus | 36 | CFAV | 0 (2) | 0 (2) | |
| College Station | A. albopictus | 9 | CFAV | 0 (3) | 0 (3) | |
| LRGV | C. quinquefasciatus | 25 | CxFLAV | 0 (2) | 0 (2) | |
| San Antonio | C. quinquefasciatus | 13 | CxFLAV | 0 (2) | 0 (2) | |
| College Station | C. quinquefasciatus | 100 | CxFLAV | 0 (2) | 2 (2) | 100 (KX512322) |
| Chicago | Culex spp. | 70 | CxFLAV | 2 (2) | 1 (2) | 100 (KX512322) |
| LRGV | Culex spp. | 16 | CxFLAV | 0 (2) | 0 (2) |
a
Number of positive pools (total number of pools tested).