TABLE 1.

Representative functional groups for differentially expressed proteins during ethanol adaptation of S. Enteritidis

Functional group or GenBank/UniProt accession no.	Descriptiona	Gene name	Ratio
Metabolism
Q4VYA5	5-Hydroxyisourate hydrolase	hiuH	1.98
B5QTE0	UPF0345 protein YaiE	yaiE	1.36
A0A1R2HTP8	Histidine biosynthesis bifunctional protein HisIE	hisI	1.34
B5QWI9	Arginine N-succinyltransferase	astA	1.81
P29848	Cysteine synthase	cysM	0.59
M7RD94	Aminotransferase, class I/II	STM1557	0.69
M7RWR3	Ketose-bisphosphate aldolase	gatY	1.52
A0A0F6AY02	Glyoxylate carboligase	gcl	1.43
A0A1R2VYV9	Transketolase protein	STM2340	1.40
EPI76970	PTS system fructose-specific EIIBBC component	fruA	1.30
B5R1U0	Ribulokinase	araB	0.72
A0A0F5BCE2	Succinate dehydrogenase hydrophobic membrane anchor subunit	sdhD	0.73
A0A1R2PZL4	PTS system glucitol/sorbitol-specific IIBC component	srlE	0.49
B5R5F8	Phosphopantetheine adenylyltransferase	coaD	1.36
P0A1A1	Thioesterase family protein	yciA	0.61
AMN27763	Penicillin binding protein	mrdA	0.73
A0A2R4HP12	NDH-1 subunit M	nuoM	1.37
B5R2Z7	NADH-quinone oxidoreductase subunit N	nuoN	1.38
AIN67540	Polyprenyl synthetase	ispA	0.69
ABC transporters
Q8ZML1	Glycine betaine-binding periplasmic protein	proX	1.65
P17328	Glycine betaine/l-proline transport ATP-binding protein ProV	proV	1.42
E8XHU4	Phosphate transport system permease protein	pstC	1.56
Q8ZKX5	Phosphate-binding protein PstS	pstS	1.76
A0A0F7J509	Thiamine/thiamine pyrophosphate ABC transporter	tbpA	1.35
A0A2R4HK85	Heme exporter protein	ccmC	1.36
EPI87418	ABC transporter, substrate-binding protein, family 3	dalS	1.31
P36638	Peptide transport system ATP-binding protein SapF	sapF	0.68
AHO69181	Manganese transport system ATP-binding protein MntA	sitB	0.71
Regulators
B5R4S3	Sigma factor-binding protein Crl	crl	1.40
B5R1C8	DNA-binding protein Fis	fis	0.52
A0A0F6B247	Regulator of RpoS	rssB	1.33
B5QV29	HTH-type transcriptional repressor PurR	purR	0.75
A0A1R2QC05	HTH-type transcriptional regulator MetR	metR	0.68
P0A9Y5	Cold shock protein CspA	cspA	0.47
Ribosome
B5QUQ1	50S ribosomal protein L34	rpmH	0.70
B5R1F9	50S ribosomal protein L30	rpmD	0.72
B4TKK2	30S ribosomal protein S14	rpsN	0.74
B5R0L7	30S ribosomal protein S9	rpsI	0.75
AIN66722	ATP-dependent RNA helicase RhlE	rhlE	0.66
A0A2R4HLT0	ATP-dependent RNA helicase DeaD	deaD	0.70
EPJ03745	ATP-dependent RNA helicase DbpA	dbpA	0.74
B5R578	GTPase Der	der	0.73
B5R0H4	GTPase Obg	obg	0.74
B5QZV7	Ribosome-binding factor A	rbfA	0.71
B5QUG2	tRNA (guanine-N1-)-methyltransferase	trmD	0.72
Enterobactin biosynthesis and uptake
AIN08681	2,3-Dihydroxybenzoate-2,3-dehydrogenase	entA	0.59
Q8ZR31	Isochorismatase	entB	0.71
AIN08678	Isochorismate synthase	entC	0.67
AIN08671	Enterobactin synthetase component F	entF	0.73
B5QVK0	Proofreading thioesterase EntH	entH	0.72
EPI62949	Colicin I receptor	cirA	0.56
V7IPX8	Ferrienterobactin receptor	fepA	0.60
B5R716	Ferrienterobactin-binding periplasmic protein	fepB	0.76
A0A0F6AY78	Achromobactin ABC transporter, ATP-binding protein	fepC	0.70
Flagella
P16323	Flagellar basal body protein	flgF	0.63
AHQ29209	Flagellar assembly protein FliH	fliH	0.75
Salmonella pathogenicity island
AIN69063	Outer membrane autotransporter barrel domain protein	misL	0.54
AIN69734	Type III secretion apparatus lipoprotein, YscJ/HrcJ family	prgK	0.68
P0A1B9	Invasion protein InvC	invC	0.69
AIN02111	Type III secretion apparatus needle protein	prgI	0.72

^aPTS, phosphotransferase; HTH, helix-turn-helix.